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Image Search Results
Journal: Virology Journal
Article Title: Identification of linear B cell epitopes on the E146L protein of African swine fever virus with monoclonal antibodies
doi: 10.1186/s12985-024-02570-0
Figure Lengend Snippet: Preliminary epitope mapping of mAbs. ( A ) The outer-membrane domain of the E146L protein was truncated into seven short peptides 30 amino acids in length with 15 amino acids overlapping. IMD: internal membrane domain, TMD: transmembrane domain. ( B ) The peptides were expressed as MBP fusion proteins in Escherichia coli and verified by SDS‒PAGE. The peptide fusion proteins were used to map the epitopes of the mAbs by ELISA ( C , E and G ) and western blotting ( D , F and H ). The results revealed that the mAb 12H12 recognized peptides P1 and P2, and the mAbs 15G1 and 15H10 identified peptides P7 and P1, respectively
Article Snippet: Six transmembrane proteins of ASFV (I329L, E146L, C257L, EP153R, I177L, and F165R) were selected through the
Techniques: Membrane, Enzyme-linked Immunosorbent Assay, Western Blot
Journal: BMC Genomics
Article Title: PP2 gene family in Phyllostachys edulis : identification, characterization, and expression profiles
doi: 10.1186/s12864-024-11007-5
Figure Lengend Snippet: Phylogenetic analysis was performed on 163 predicted PP2 protein sequences from eight plant species. Each subfamily represented a distinct color. The prefixes Phe, Ola, Rgu, Rdi, Bam, Gan, At, and Osa were used to identify PP2 proteins from P. edulis , O. latifolia , R. guianensis , R. distichophylla , B. amplexicaulis , G. angustifolia , A. thaliana , and O. sativa , respectively. RAxML-NG on ACCESS (1.2.0) in CIPRES ( https://www.phylo.org/ ) was used to construct a maximum likelihood (ML) phylogenetic tree. Furthermore, TBtools v2.110 was used for visualization
Article Snippet: A hidden Markov model file of
Techniques: Construct